Fig 1: LINC00707 promotes CC cell proliferation via the miR-374c-5p/SDC4 axis. (a) Sh-SDC4#1/#2 transfection in the SiHa cell line to knock down gene expression. (b, c) The viability and proliferation of CC cells were measured by CCK-8 along with colony formation assay in different groups (sh-NC, sh-LINC00707#1, sh-LINC00707#1+miR-374c-5p inhibitor, and sh-LINC00707#1+miR-374c-5p inhibitor+sh-SDC4#1). (d, e) Cell apoptosis change was measured by TUNEL and western blot assays. GAPDH was an internal control. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01.
Fig 2: SDC4 is the target gene of miR-374c-5p in CC cells. (a) Potential mRNAs that may bind to miR-374c-5p were selected using starBase. (b) RNA pull-down assay was taken to measure the enrichment of selected two mRNAs (SDC4 and ARID4A) in Bio-miR-374c-5p-WT/Mut groups. (c) The identified SDC4 expression in CC cell lines. (d) RIP assay was performed to test the enrichment of LINC00707, miR-374c-5p, and SDC4 in the anti-Ago2 group and the control IgG group. (e) miR-374c-5p expression was elevated in the SiHa cell line via miR-374c-5p mimic transfection while it was reduced via miR-374c-5p inhibitor transfection in C-33A cells. (f) The relative expression along with protein level of SDC4 upon miR-374c-5p overexpression or inhibition. (g) The binding sites between miR-374c-5p and SDC4 were evidenced by the luciferase reporter assay. (h) SDC4 expression in sh-LINC00707#1-transfected CC cells. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.
Supplier Page from Abcam for Human Syndecan-4 ELISA Kit (SDC4)